Standardization of PCR technique for detecting Listeria monocytogenes in chicken, beef and pork

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dc.creatorASAEL EDEM DE LA ROSA ZARIÑANA
dc.creator.fourMIGUEL ANGEL MATA ESPINOSA
dc.creator.idinfo:eu-repo/dai/mx/cvu/589612
dc.creator.idfourinfo:eu-repo/dai/mx/cvu/101496
dc.creator.idoneinfo:eu-repo/dai/mx/cvu/66003
dc.creator.idthreeinfo:eu-repo/dai/mx/cvu/25902
dc.creator.idtwoinfo:eu-repo/dai/mx/cvu/3171
dc.creator.oneMARIA MAGDALENA CROSBY GALVAN
dc.creator.threeDAVID HERNANDEZ SANCHEZ
dc.creator.twoMARTA ELVA RAMIREZ GUZMAN
dc.date.accessioned2018-04-10T01:28:44Z
dc.date.available2018-04-10T01:28:44Z
dc.date.issued2017-08-14
dc.descriptionEl objetivo fue estandarizar la técnica de PCR para el diagnóstico de Listeria monocytogenes en carne de pollo, res y cerdo. Se evaluó la sensibilidad y especi cidad de la PCR y el nivel de concordancia con el método microbiol ógico. La PCR se estandarizó utilizando un total de 60 muestras de carne de pollo, res y cerdo, (20 muestras por tipo de carne).
dc.description.abstractThe aim of this study was to standardize the PCR technique for detecting Listeria monocytogenes (Lm) in chicken, beef and pork. The sensitivity and speci city of PCR and the level of concordance with the microbiological method were evaluated. PCR was standardized using a total of 60 samples of chicken, beef and pork (20 samples per meat type.) Sensitivity and speci city were calculated using the 2 x 2 table and the level of accordance by the Kappa index. The minimum detectable DNA concentration was 3.0 ng L1. The PCR showed 100% sensitivity and speci city, and 80, 91 and 100% concordance between the methods was obtained for detecting Lm in chicken, beef and pork samples respectively, with 89.4% similarity between the methods for the three types of meat.
dc.format1
dc.identifier.urihttps://ri.ujat.mx/handle/20.500.12107/976
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/openAccesses_MX
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0es_MX
dc.subject.ctiinfo:eu-repo/classification/cti/6
dc.subject.keywordsListeria monocytogenes
dc.subject.keywordsPCR
dc.subject.keywordsmeat
dc.subject.keywordsvalidation
dc.subject.keywordssensitivity
dc.subject.keywordsBayes' Theorem
dc.subject.keywordsKappa index
dc.titleStandardization of PCR technique for detecting Listeria monocytogenes in chicken, beef and pork
dc.typeinfo:eu-repo/semantics/article

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Vol. 5, Núm. 13 (2018)