Introgresión de eventos transgénicos y secuencias acompañantes en maíces mexicanos

Abstract

Representative seed samples of 215 of maize varieties from four(Jalisco, Michoacán, Oaxaca and Puebla) Mexican States were collected. The totalgenotypes collected in each State were 53, 96, 46 and 20 samples, respectively.Each seed collection was geo-referenced, then seed was planted and leaf tissuewas used for DNA isolation, after that a PCR analysis was performed to determinethe presence or absence of eight accompanying sequences and nine transgenicevents in the 215 maize genotypes. Results showed introgression of accompanyingsequences and transgenic events (cry1Ab) into some maize varieties. The majorproportion ofcry1Abresidues was observed in maize samples from Puebla (70%)followed by that displayed in the Oaxaca samples (52%), while Jalisco samplesshowed 15 and 7.5% of thecry1Abgene andntpIIaccompanying sequence,respectively, and Michoacán samples exhibited 44 and 17.7% forcry1Abgene andthe accompanying sequence, respectively. These results supported for applicatecorrective measures for to prevent the genetic contamination of Mexican maizevarieties and to clean up out-of-type phenotypes that could be sources of contami-nation of transgenic genes within maize populations contaminated with transgenes.